Disassembly of intact multiprotein complexes in the gas phase.

Journal article

The observation of multiprotein complexes by mass spectrometry formerly relied upon chemical cross-linking to maintain interactions. Recent technological developments have enabled the observation of intact macromolecular complexes without modification. These assemblies, with masses far in excess of those measured previously, can be examined through controlled dissociation in the mass spectrometer, revealing information about their subunit interactions and topology.

Authors: Rostom, A; Robinson, C

• Journal: Current opinion in structural biology
• Volume: 9
• Issue: 1
• Pages: 135-141
• Publication date: 1999-02-01
• DOI: 10.1016/s0959-440x(99)80018-9
• EISSN: 1879-033X
• ISSN: 0959-440X
• Language: English
• Keywords: Protein Conformation; Ribosomal Proteins; Escherichia coli; Retinol-Binding Proteins; Bacterial Proteins; Proteins; Models, Molecular; Mass Spectrometry; Prealbumin; Macromolecular Substances; Gases