Electroporation-induced siRNA precipitation obscures the efficiency of siRNA loading into extracellular vesicles.

Journal article

Extracellular vesicles (EVs) are specialised endogenous carriers of proteins and nucleic acids and are involved in intercellular communication. EVs are therefore proposed as candidate drug delivery systems for the delivery of nucleic acids and other macromolecules. However, the preparation of EV-based drug delivery systems is hampered by the lack of techniques to load the vesicles with nucleic acids. In this work we have now characterised in detail the use of an electroporation method for thi...

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Authors: Kooijmans, SA; Stremersch, S; Braeckmans, K; de Smedt, S; Hendrix, A

• Publication status: Published
• Journal: Journal of controlled release : official journal of the Controlled Release Society
• Volume: 172
• Issue: 1
• Pages: 229-238
• Publication date: 2013-11-01
• DOI: 10.1016/j.jconrel.2013.08.014
• EISSN: 1873-4995
• ISSN: 0168-3659
• Language: English
• Keywords: Humans; Chemical Precipitation; Solubility; Edetic Acid; Exosomes; Drug Carriers; Electroporation; HEK293 Cells; RNA, Small Interfering