Journal article
Hypoxia-activated small molecule-induced gene expression
- Abstract:
- Hypoxia, a condition of reduced oxygen, occurs in a wide variety of biological contexts, including solid tumors and bacterial biofilms, which are relevant to human health. Consequently, the development of chemical tools to study hypoxia is vital. Here we report a hypoxia-activated, small-molecule-mediated gene expression system using a bioreductive prodrug of the inducer isopropyl 1-thio-β-d-galactopyranoside. As a proof-of-concept we have placed the production of a green fluorescent protein under the control of hypoxia. Our system has the potential to be extended to regulate the production of any given protein of choice.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 3.0MB, Terms of use)
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- Publisher copy:
- 10.1021/acschembio.8b00858
Authors
+ Novartis
More from this funder
- Funding agency for:
- Collins, S
- Hammond, E
- Conway, S
- Grant:
- BB/L016214/1
- C5255/A23755
- BB/L016214/1
+ Biotechnology and Biological Sciences Research Council
More from this funder
- Funding agency for:
- Collins, S
- Conway, S
- Grant:
- BB/L016214/1
- BB/L016214/1
- Publisher:
- American Chemical Society
- Journal:
- ACS Chemical Biology More from this journal
- Volume:
- 13
- Issue:
- 12
- Pages:
- 3354–3360
- Publication date:
- 2018-11-19
- Acceptance date:
- 2018-11-19
- DOI:
- EISSN:
-
1554-8937
- ISSN:
-
1554-8929
- Language:
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English
- Pubs id:
-
pubs:948829
- UUID:
-
uuid:b1e88f04-750e-4110-968a-a71aca0dbbaf
- Local pid:
-
pubs:948829
- Source identifiers:
-
948829
- Deposit date:
-
2018-11-30
Terms of use
- Copyright holder:
- American Chemical Society
- Copyright date:
- 2018
- Notes:
- Copyright © 2018 American Chemical Society. This is the accepted manuscript version of the article. The final version is available online from the American Chemical Society at: https://doi.org/10.1021/acschembio.8b00858
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