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Journal article

Increase in copy number of an integrated vector during continuous culture of Hansenula polymorpha expressing functional human haemoglobin.

Abstract:

Recombinant human haemoglobin A (rHbA) was produced by a leucine-requiring strain of Hansenula polymorpha which had been transformed with an integration vector containing the Saccharomyces cerevisiae LEU2 gene and cDNAs for the expression of alpha and beta globin each driven by the H. polymorpha MOX promoter. After 40 generations in a chemostat it was found that the integrated vector had become amplified in the host strain. In some cases this led to an increase in LEU2 gene dosage, but a loss...

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Publication status:
Published

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Publisher copy:
10.1002/yea.320101206

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Jenner Institute
Role:
Author
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Journal:
Yeast (Chichester, England)
Volume:
10
Issue:
12
Pages:
1569-1580
Publication date:
1994-12-05
DOI:
EISSN:
1097-0061
ISSN:
0749-503X
URN:
uuid:b12ae37c-f429-4a9d-844c-9f18768a2fbd
Source identifiers:
93485
Local pid:
pubs:93485

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