Hydroxy-terminated conjugated polymer nanoparticles have near-unity bright fraction and reveal cholesterol-dependence of IGF1R nanodomains.

Journal article

Fluorescent nanoparticles have enabled many discoveries regarding how molecular machines function. Quantum dots have been the dominant class of fluorescent nanoparticles but suffer from blinking and from a substantial dark fraction--particles where the fluorescence is never seen--complicating any analysis of biological function. Nanoparticles composed of conjugated fluorescent polymers (Pdots) have recently been shown to have high brightness and no blinking. Here we develop a robust and effic...

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Authors: Koner, A; Krndija, D; Hou, Q; Sherratt, D; Howarth, M

• Publication status: Published
• Journal: ACS nano
• Volume: 7
• Issue: 2
• Pages: 1137-1144
• Publication date: 2013-02-01
• DOI: 10.1021/nn3042122
• EISSN: 1936-086X
• ISSN: 1936-0851
• Language: English
• Keywords: Protein Structure, Tertiary; Models, Molecular; Fluorescent Dyes; Cholesterol; Receptor, IGF Type 1; Cell Line, Tumor; Nanoparticles; Darkness; MCF-7 Cells; Polymers; Humans; Movement; Cell Membrane