Tetradecanoylphorbol-13-acetate (TPA) significantly increases AAV2/5 transduction of human neuronal cells in vitro.

Journal article

Recombinant adeno-associated virus type 2 (AAV2) vectors have shown great promise in current ophthalmology clinical trials targeting gene delivery to the retinal pigment epithelium (RPE). To treat the majority of retinal diseases, however, gene delivery would need to be targeted to photoreceptor neurons of the outer retina. AAV2 pseudotyped with the AAV5 capsid (AAV2/5) has shown far greater transduction efficiency in photoreceptors compared to standard AAV2 vectors. For clinical trial applic...

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Authors: You, Q; Brown, L; McClements, M; Hankins, M; Maclaren, R

• Publication status: Published
• Journal: Experimental eye research
• Volume: 97
• Issue: 1
• Pages: 148-153
• Publication date: 2012-04-01
• DOI: 10.1016/j.exer.2011.12.009
• EISSN: 1096-0007
• ISSN: 0014-4835
• Language: English
• Keywords: RNA, Messenger; Retinal Neoplasms; Receptors, Platelet-Derived Growth Factor; Cell Division; Dependovirus; DNA-Binding Proteins; Tretinoin; Tetradecanoylphorbol Acetate; Transduction, Genetic; Gene Expression Regulation; Tumor Cells, Cultured; Cell Cycle Proteins; Retinoblastoma; Real-Time Polymerase Chain Reaction; Tacrolimus Binding Proteins; HEK293 Cells; Humans; Tumor Suppressor Proteins; Carcinogens; Green Fluorescent Proteins; Protein-Serine-Threonine Kinases; Cell Differentiation; Genetic Vectors