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Functional and structural characterization of the Methanosarcina mazei proteasome and PAN complexes.

Abstract:

We have cloned the proteasome and the proteasome activating nucleotidase (PAN) genes from the mesophilic archaeon Methanosarcina mazei and produced the respective proteins in Escherichia coli cultures. The recombinant complexes were purified to homogeneity and characterized biochemically, structurally, and by mass spectrometry. We found that the degradation of Bodipy-casein by Methanosarcina proteasomes was activated by Methanosarcina PAN. Notably, the Methanosarcina PAN unfolded GFP-SsrA onl...

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Publication status:
Published

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Publisher copy:
10.1016/j.jsb.2006.03.015

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Volume:
156
Issue:
1
Pages:
84-92
Publication date:
2006-10-05
DOI:
EISSN:
1095-8657
ISSN:
1047-8477
URN:
uuid:b0a7b699-8121-4793-9190-31844f8d7da6
Source identifiers:
59422
Local pid:
pubs:59422

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