Conference item icon

Conference item

Functional and structural characterization of the Methanosarcina mazei proteasome and PAN complexes.

Abstract:

We have cloned the proteasome and the proteasome activating nucleotidase (PAN) genes from the mesophilic archaeon Methanosarcina mazei and produced the respective proteins in Escherichia coli cultures. The recombinant complexes were purified to homogeneity and characterized biochemically, structurally, and by mass spectrometry. We found that the degradation of Bodipy-casein by Methanosarcina proteasomes was activated by Methanosarcina PAN. Notably, the Methanosarcina PAN unfolded GFP-SsrA onl...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1016/j.jsb.2006.03.015

Authors


Expand authors...
Volume:
156
Issue:
1
Pages:
84-92
Host title:
Journal of structural biology
Publication date:
2006-10-01
Event location:
United States
DOI:
EISSN:
1095-8657
ISSN:
1047-8477
Source identifiers:
59422

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP