Evidence for cross-linking DNA by bis-intercalators with rigid and extended linkers is provided by knotting and catenation.

Journal article

A new series of DNA bis-intercalators is reported in which acridine moieties are connected by rigid and extended pyridine-based linkers of varied length. Cross-linking of DNA by bis-intercalation is inferred from the unwinding and folding of linear DNA induced by the compounds; after ligation and removal of the bis-intercalator, superhelical circles, catenanes and knots that bear a residual imprint of the bis-intercalator are observed. These novel bis-intercalators are of interest because the...

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Authors: Annan, N; Cook, P; Mullins, S; Lowe, G

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 20
• Issue: 5
• Pages: 983-990
• Publication date: 1992-03-01
• DOI: 10.1093/nar/20.5.983
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: Intercalating Agents; Cloning, Molecular; DNA, Superhelical; Magnetic Resonance Spectroscopy; Humans; Acridine Orange; Hela Cells; Electrophoresis; Cell Division; Nucleic Acid Conformation; Ethidium