- Abstract:
-
The bcl-2 proto-oncogene, rearranged and deregulated in B-cell lymphomas bearing the t(14;18) translocation, encodes an inner mitochondrial membrane protein that blocks apoptotic cell death. We have developed a sensitive immunofluorescence assay for the single- and multicolor flow cytometric analysis of bcl-2 protein in relation to other markers and cell cycle, based on a fixation-permeation step of cells with paraformaldehyde and Triton X100 and the use of a bcl-2 specific monoclonal antibod...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1002/cyto.990130509
-
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- Journal:
- Cytometry
- Volume:
- 13
- Issue:
- 5
- Pages:
- 502-509
- Publication date:
- 1992-01-01
- DOI:
- EISSN:
-
1097-0320
- ISSN:
-
0196-4763
- URN:
-
uuid:b05cc914-929b-4b8b-b6fd-7269b6201358
- Source identifiers:
-
486742
- Local pid:
- pubs:486742
- Language:
- English
- Keywords:
- Copyright date:
- 1992
Journal article
Flow cytometric detection of the mitochondrial BCL-2 protein in normal and neoplastic human lymphoid cells.
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