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Journal article

Flow cytometric detection of the mitochondrial BCL-2 protein in normal and neoplastic human lymphoid cells.

Abstract:

The bcl-2 proto-oncogene, rearranged and deregulated in B-cell lymphomas bearing the t(14;18) translocation, encodes an inner mitochondrial membrane protein that blocks apoptotic cell death. We have developed a sensitive immunofluorescence assay for the single- and multicolor flow cytometric analysis of bcl-2 protein in relation to other markers and cell cycle, based on a fixation-permeation step of cells with paraformaldehyde and Triton X100 and the use of a bcl-2 specific monoclonal antibod...

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Publication status:
Published

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Publisher copy:
10.1002/cyto.990130509

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Journal:
Cytometry
Volume:
13
Issue:
5
Pages:
502-509
Publication date:
1992-01-01
DOI:
EISSN:
1097-0320
ISSN:
0196-4763
URN:
uuid:b05cc914-929b-4b8b-b6fd-7269b6201358
Source identifiers:
486742
Local pid:
pubs:486742

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