Extracellular ATP increases cytoplasmic free Ca2+ concentration in clonal insulin-producing RINm5F cells. A mechanism involving direct interaction with both release and refilling of the inositol 1,4,5-trisphosphate-sensitive Ca2+ pool.

Journal article

Effects of extracellularly applied ATP (added as disodium salt) on stimulus-secretion coupling were investigated in clonal insulin-producing RINm5F cells. Cytoplasmic free Ca2+ concentration [( Ca2+]i), electrical activity, membrane potential, formation of InsP3 and insulin release were measured. Addition of ATP in a Ca2(+)-containing medium promoted a rapid rise in [Ca2+]i, which was followed by a slow decline towards the basal level. In a Ca2(+)-free medium, the ATP-induced increase in [Ca2...

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Authors: Arkhammar, P; Hallberg, A; Kindmark, H; Nilsson, T; Rorsman, P

• Publication status: Published
• Journal: Biochemical journal
• Volume: 265
• Issue: 1
• Pages: 203-211
• Publication date: 1990-01-01
• EISSN: 1470-8728
• ISSN: 0264-6021
• Language: English
• Keywords: Membrane Potentials; Indicators and Reagents; Cell Membrane Permeability; Adenosine Triphosphate; Calcium; Cell Line; Ethidium; Inositol Phosphates; Insulin; Cytoplasm