Mass measurements of increased accuracy resolve heterogeneous populations of intact ribosomes.

Journal article

It is established that noncovalent complexes can be maintained both during and after electrospray and that assemblies of increasing size and complexity often lead to broadened peaks in mass spectra. This broadening arises from the tendency of large protein assemblies to form adducts with salts and is compounded when complexes are isolated directly from cells, without the full protein complement. To investigate the origins of this broadening in mass spectral peaks and to develop the optimal me...

Expand abstract

Authors: McKay, A; Ruotolo, B; Ilag, L; Robinson, C

• Publication status: Published
• Journal: Journal of the American Chemical Society
• Volume: 128
• Issue: 35
• Pages: 11433-11442
• Publication date: 2006-09-01
• DOI: 10.1021/ja061468q
• EISSN: 1520-5126
• ISSN: 0002-7863
• Language: English
• Keywords: Tandem Mass Spectrometry; Molecular Weight; Spectrometry, Mass, Electrospray Ionization; Ribosomal Proteins; Ribosomes; Sensitivity and Specificity; Thermus thermophilus; Protein Subunits; Models, Molecular