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Random PCR and ultracentrifugation increases sensitivity and throughput of VIDISCA for screening of pathogens in clinical specimens.

Abstract:

INTRODUCTION: Virus discovery based on cDNA-AFLP (VIDISCA) is a sequence-independent virus discovery method that was recently developed and successfully used to characterize unknown viruses in cell cultures. Its applicability, however, is limited by its low sensitivity. METHODOLOGY: We evaluated whether the introduction of prior amplification of target sequences by random PCR (rPCR) increases the sensitivity of this method to improve its use on clinical specimens. In addition, ultracentrifug...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Tropical Medicine
Van der Hoek, L More by this author
Minh Hien, V More by this author
Jebbink, MF More by this author
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Journal:
Journal of infection in developing countries
Volume:
5
Issue:
2
Pages:
142-148
Publication date:
2011-02-05
EISSN:
1972-2680
ISSN:
2036-6590
URN:
uuid:afd1aabd-0040-401d-8556-4cbae9a4f8db
Source identifiers:
124344
Local pid:
pubs:124344

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