The use of monoclonal antibodies UCH beta and UCH gamma for the antenatal diagnosis of beta-thalassaemia.

Journal article

Monoclonal antibodies for the beta-globin chain of human HbA (UCH beta) and the gamma-globin chain of human HbF (UCH gamma) have been made. UCH beta indirectly labelled with rhodamine-labelled goat anti-mouse Ig and directly flouresceinated UCH gamma have been used, via double labelling immunofluorescence microscopy, to assay for the presence of beta-chains in fetal erythrocytes obtained at fetoscopy from fetuses at risk for beta-thalassaemia major. The results from 111 cases demonstrate that...

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Authors: Allen, C; Weereratne, H; Gale, R; Beverley, P; Huehns, E

• Journal: British journal of haematology
• Volume: 65
• Issue: 2
• Pages: 199-203
• Publication date: 1987-02-01
• DOI: 10.1111/j.1365-2141.1987.tb02265.x
• EISSN: 1365-2141
• ISSN: 0007-1048
• Language: English
• Keywords: Female; Fluorescent Antibody Technique; Pregnancy; Prenatal Diagnosis; Hemoglobin A; Antibody Specificity; Fetal Hemoglobin; Fetal Diseases; Humans; Antibodies, Monoclonal; Thalassemia