Journal article icon

Journal article

Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot.

Abstract:

The ELISpot assay is used in vaccine studies for the quantification of antigen-specific memory B cells (B(MEM)), and can be performed using cryopreserved samples. The effects of cryopreservation on B(MEM) detection and the consistency of cultured ELISpot assays when performed by different operators or laboratories are unknown. In this study, blood was taken from healthy volunteers, and a cultured ELISpot assay was used to count B(MEM) specific for 2 routine vaccine antigens (diphtheria and te...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.4161/hv.29318

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Paediatrics
Mitchell, R More by this author
Thompson, AJ More by this author
Morales-Aza, B More by this author
Clutterbuck, EA More by this author
Expand authors...
Journal:
Human vaccines and immunotherapeutics
Volume:
10
Issue:
8
Pages:
2490-2496
Publication date:
2014-01-05
DOI:
EISSN:
2164-554X
ISSN:
2164-5515
URN:
uuid:afa2fd2c-c427-4d88-ac76-08c2259aa48e
Source identifiers:
489277
Local pid:
pubs:489277

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP