Positive modulation of RNA polymerase III transcription by ribosomal proteins.

Journal article

A yeast nuclear fraction of unknown composition, named TFIIIE, was reported previously to enhance transcription of tRNA and 5S rRNA genes in vitro. We show that TFIIIE activity co-purifies with a specific subset of ribosomal proteins (RPs) which, as revealed by chromatin immunoprecipitation analysis, generally interact with tRNA and 5S rRNA genes, but not with a Pol II-specific promoter. Only Rpl6Ap and Rpl6Bp, among the tested RPs, were found associated to a TATA-containing tRNA(Ile)(TAT) ge...

Expand abstract

Authors: Dieci, G; Ruotolo, R; Braglia, P; Carles, C; Carpentieri, A

• Publication status: Published
• Journal: Biochemical and biophysical research communications
• Volume: 379
• Issue: 2
• Pages: 489-493
• Publication date: 2009-02-01
• DOI: 10.1016/j.bbrc.2008.12.097
• EISSN: 1090-2104
• ISSN: 0006-291X
• Language: English
• Keywords: Transcription Factors, TFIII; RNA Polymerase III; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Gene Expression Regulation, Fungal; RNA, Ribosomal, 5S; Ribosomal Proteins; Mutation; Transcription, Genetic; Promoter Regions, Genetic; Chromatin Immunoprecipitation; RNA, Transfer, Ile