The after-hours mutant reveals a role for Fbxl3 in determining mammalian circadian period.

Journal article

By screening N-ethyl-N-nitrosourea-mutagenized animals for alterations in rhythms of wheel-running activity, we identified a mouse mutation, after hours (Afh). The mutation, a Cys(358)Ser substitution in Fbxl3, an F-box protein with leucine-rich repeats, results in long free-running rhythms of about 27 hours in homozygotes. Circadian transcriptional and translational oscillations are attenuated in Afh mice. The Afh allele significantly affected Per2 expression and delayed the rate of Cry prot...

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Authors: Godinho, S; Maywood, E; Shaw, L; Tucci, V; Barnard, A

• Publication status: Published
• Journal: Science (New York, N.Y.)
• Volume: 316
• Issue: 5826
• Pages: 897-900
• Publication date: 2007-05-01
• DOI: 10.1126/science.1141138
• EISSN: 1095-9203
• ISSN: 0036-8075
• Language: English
• Keywords: Lung; Gene Expression Regulation; Animals; Transcription, Genetic; Cryptochromes; Circadian Rhythm; ARNTL Transcription Factors; Male; Trans-Activators; Basic Helix-Loop-Helix Transcription Factors; Mice, Inbred BALB C; Cell Cycle Proteins; Amino Acid Sequence; Molecular Sequence Data; Point Mutation; Mice; CLOCK Proteins; Transcription Factors; COS Cells; Amino Acid Substitution; Suprachiasmatic Nucleus; Period Circadian Proteins; Liver; Crosses, Genetic; Female; Flavoproteins; Nuclear Proteins; Mice, Inbred C3H; Cercopithecus aethiops; F-Box Proteins