Identification of trapped and boundary lipid binding sites in M13 coat protein/lipid complexes by deuterium NMR spectroscopy.

Journal article

The major coat protein of M13 bacteriophage has been incorporated into bilayers of 1,2-dimyristoyl-sn-glycero-3-phosphocholine, deuterated in the trimethyl segments of the choline headgroup (DMPC-d9). Two-component deuterium and phosphorus-31 NMR spectra have been observed from bilayer complexes containing the coat protein, indicating slow exchange (on the deuterium quadrupole anisotropy and phosphorus-31 chemical shift averaging time scales) of lipid molecules of less than 10(3) Hz between t...

Expand abstract

Authors: Van Gorkom, L; Horváth, L; Hemminga, M; Sternberg, B; Watts, A

• Publication status: Published
• Journal: Biochemistry
• Volume: 29
• Issue: 16
• Pages: 3828-3834
• Publication date: 1990-04-01
• DOI: 10.1021/bi00468a004
• EISSN: 1520-4995
• ISSN: 0006-2960
• Language: English
• Keywords: Binding Sites; Lipid Bilayers; Magnetic Resonance Spectroscopy; Capsid; Dimyristoylphosphatidylcholine; Bacteriophages; Deuterium; Membrane Proteins; Viral Matrix Proteins; Capsid Proteins