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In-depth quantitative cardiac proteomics combining electron transfer dissociation and the metalloendopeptidase Lys-N with the SILAC mouse

Abstract:

In quantitative proteomics stable isotope labeling has progressed from cultured cells toward the total incorporation of labeled atoms or amino acids into whole multicellular organisms. For instance, the recently introduced 13C 6-lysine labeled SILAC mouse allows accurate comparison of protein expression directly in tissue. In this model, only lysine, but not arginine, residues are isotope labeled, as the latter may cause complications to the quantification by in vivo conversion of arginine to...

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Publisher copy:
10.1074/mcp.O111.008474

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Role:
Author
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Journal:
Molecular and Cellular Proteomics
Volume:
10
Issue:
10
Publication date:
2011-10-05
DOI:
EISSN:
1535-9484
ISSN:
1535-9476
URN:
uuid:aebcceb5-0c9e-4480-a364-49b472854566
Source identifiers:
385456
Local pid:
pubs:385456
Language:
English

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