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Peptides derived from the auxin binding protein elevate Ca2+ and pH in stomatal guard cells of Vicia faba: a confocal fluorescence ratio imaging study.

Abstract:

Dual-excitation confocal laser scanning microscopy (CLSM) was used to image the pH-indicator, BCECF, iontophoretically microinjected into stomatal guard cells of Vicia faba during challenge with peptides derived from hydrophilic domains of the maize auxin-binding protein. Only the peptide corresponding to the C-terminal end (Pz151-163) caused significant changes in cytosolic pH, stimulating rapid alkalinisation of 0.4 +/- 0.1 pH units. Cytosolic pH was clamped using the permeant weak acid, bu...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
Volume:
48
Issue:
98
Pages:
215-228
Host title:
Symposia of the Society for Experimental Biology
Publication date:
1994-01-01
Event location:
ENGLAND
ISSN:
0081-1386
Source identifiers:
44314
ISBN:
0948601485

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