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Peptides derived from the auxin binding protein elevate Ca2+ and pH in stomatal guard cells of Vicia faba: a confocal fluorescence ratio imaging study.

Abstract:

Dual-excitation confocal laser scanning microscopy (CLSM) was used to image the pH-indicator, BCECF, iontophoretically microinjected into stomatal guard cells of Vicia faba during challenge with peptides derived from hydrophilic domains of the maize auxin-binding protein. Only the peptide corresponding to the C-terminal end (Pz151-163) caused significant changes in cytosolic pH, stimulating rapid alkalinisation of 0.4 +/- 0.1 pH units. Cytosolic pH was clamped using the permeant weak acid, bu...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Millner, P More by this author
Volume:
48
Issue:
98
Pages:
215-228
Publication date:
1994
ISSN:
0081-1386
URN:
uuid:ae205383-53a5-409e-b60e-1077e8116e2e
Source identifiers:
44314
Local pid:
pubs:44314
ISBN:
0-948601-48-5

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