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In vivo characterization of regulatory polymorphisms by allele-specific quantification of RNA polymerase loading.

Abstract:

In vivo characterization of regulatory polymorphisms is a key requirement for next-generation human genetic analysis. Here we describe haploChIP, a method that uses chromatin immunoprecipitation (ChIP) and mass spectrometry to identify differential protein-DNA binding in vivo associated with allelic variants of a gene. We demonstrate this approach with the imprinted gene SNRPN. HaploChIP showed close correlation between the level of bound phosphorylated RNA polymerase II at the SNRPN locus an...

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Publication status:
Published

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Publisher copy:
10.1038/ng1124

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
Journal:
Nature genetics More from this journal
Volume:
33
Issue:
4
Pages:
469-475
Publication date:
2003-04-01
DOI:
EISSN:
1546-1718
ISSN:
1061-4036

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