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Journal article

RecBC, sbcB independent, (AT)n-mediated deletion of sequences flanking a Xenopus laevis beta globin gene on propagation in E. coli.

Abstract:

Plasmids containing sequences 3' of the adult beta 1 globin gene of Xenopus laevis are unstable on propagation in a range of E. coli host strains. Up to 300 bp of Xenopus DNA are lost by rec A independent recombination between (AT)37 and (AT)17 sequences. Additionally, smaller deletions occurring in or around the (AT)37 sequence are observed. Deletion of these potential cruciform structures occurs in the absence of exonuclease I, exonuclease V and exonuclease VIII as the same pattern of delet...

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Publication status:
Published

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Publisher copy:
10.1093/nar/14.10.4147

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Nucleic acids research More from this journal
Volume:
14
Issue:
10
Pages:
4147-4158
Publication date:
1986-05-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Language:
English
Keywords:
Pubs id:
pubs:8081
UUID:
uuid:ac8be1c2-7bce-483d-a4d2-5a921bb1b8a9
Local pid:
pubs:8081
Source identifiers:
8081
Deposit date:
2012-12-19

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