Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides.

Journal article

We present a straightforward method to enrich phosphopeptides with multiple basic residues, an under-represented class in common enrichment strategies. Our method is based on a two-dimensional strong cation exchange (SCX) strategy, operating at two different acidic pHs, enabling both separation and enrichment of different classes of phosphopeptides. The principle of enrichment is based on the change of net charge of phosphorylated peptides under strong acidic conditions in the second SCX, whe...

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Authors: Hennrich, M; van den Toorn, H; Groenewold, V; Heck, A; Mohammed, S

• Publication status: Published
• Journal: Analytical chemistry
• Volume: 84
• Issue: 4
• Pages: 1804-1808
• Publication date: 2012-02-01
• DOI: 10.1021/ac203303t
• EISSN: 1520-6882
• ISSN: 0003-2700
• Language: English
• Keywords: Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Hela Cells; Phosphopeptides; Chromatography, Ion Exchange; Chromatography, Liquid; Humans; Cations; Tandem Mass Spectrometry