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Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides.

Abstract:

We present a straightforward method to enrich phosphopeptides with multiple basic residues, an under-represented class in common enrichment strategies. Our method is based on a two-dimensional strong cation exchange (SCX) strategy, operating at two different acidic pHs, enabling both separation and enrichment of different classes of phosphopeptides. The principle of enrichment is based on the change of net charge of phosphorylated peptides under strong acidic conditions in the second SCX, whe...

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Publication status:
Published

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Publisher copy:
10.1021/ac203303t

Authors


Hennrich, ML More by this author
van den Toorn, HW More by this author
Groenewold, V More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Journal:
Analytical chemistry
Volume:
84
Issue:
4
Pages:
1804-1808
Publication date:
2012-02-05
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
URN:
uuid:ac0ec007-2a6f-455a-b4e5-45de77274162
Source identifiers:
384556
Local pid:
pubs:384556

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