Journal article icon

Journal article

Mimotope identification from monoclonal antibodies of Burkholderia pseudomallei using random peptide phage libraries.

Abstract:

This study used random peptide libraries, displayed by bacteriophage T7 and M13, to identify mimotopes from four monoclonal antibodies (mAbs) specific to Burkholderia pseudomallei. Bound phages, selected from fourth-round panning with each mAb, were tested for binding specificity with each mAb using ELISA, before being further amplified and checked for phage peptide sequence using PCR and DNA sequencing. Overall, 75 of 90 phages (83.3%) were ELISA-positive with each mAb. Mimotopes from all 75...

Expand abstract

Actions


Access Document


Authors


Expand authors...
Journal:
Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume:
102 Suppl 1
Issue:
SUPPL. 1
Pages:
S47-S54
Publication date:
2008-12-05
DOI:
EISSN:
1878-3503
ISSN:
0035-9203
URN:
uuid:abc63e83-9cb8-4576-b668-bb4a822b42b5
Source identifiers:
133596
Local pid:
pubs:133596

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP