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Novel homogenous time-resolved fluorometric RT-PCR assays for quantification of PSA and hK2 mRNAs in blood.

Abstract:

OBJECTIVES: The purpose of this study was to design, validate, and optimize internally standardized real-time quantitative RT-PCR assays and to identify and avoid problems with assay reliability and examine the impact of an exogenous internal standard. DESIGN AND METHODS: The model system consisted of internally standardized quantitative real-time RT-PCR assays specific for PSA and hK2 mRNA based on time-resolved fluorometric detection of lanthanide chelates. RESULTS: Reproducibility was bes...

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Authors


Rissanen, M More by this author
Väänänen, RM More by this author
Wahlroos, V More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Surgical Sciences
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Journal:
Clinical biochemistry
Volume:
40
Issue:
1-2
Pages:
111-118
Publication date:
2007-01-05
DOI:
EISSN:
1873-2933
ISSN:
0009-9120
URN:
uuid:abadb7f6-6f88-4f81-8512-df6f026a5ddf
Source identifiers:
365953
Local pid:
pubs:365953

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