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Novel homogenous time-resolved fluorometric RT-PCR assays for quantification of PSA and hK2 mRNAs in blood.

Abstract:

OBJECTIVES: The purpose of this study was to design, validate, and optimize internally standardized real-time quantitative RT-PCR assays and to identify and avoid problems with assay reliability and examine the impact of an exogenous internal standard. DESIGN AND METHODS: The model system consisted of internally standardized quantitative real-time RT-PCR assays specific for PSA and hK2 mRNA based on time-resolved fluorometric detection of lanthanide chelates. RESULTS: Reproducibility was bes...

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Institution:
University of Oxford
Division:
MSD
Department:
Surgical Sciences
Role:
Author
Journal:
Clinical biochemistry
Volume:
40
Issue:
1-2
Pages:
111-118
Publication date:
2007-01-01
DOI:
EISSN:
1873-2933
ISSN:
0009-9120
Source identifiers:
365953

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