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Journal article

The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting rotavirus A and norovirus.

Abstract:

Rotavirus (RoV) and Norovirus (NoV) are the main causes of viral gastroenteritis. Currently, there is no validated multiplex real-time PCR that can detect and quantify RoV and NoV simultaneously. The aim of the study was to develop, validate, and internally control a multiplex one-step RT real-time PCR to detect and quantify RoV and NoV in stool samples. PCR sensitivity was assessed by comparing amplification against the current gold standard, enzyme immunoassay (EIA), on stool samples from 9...

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Publication status:
Published

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Journal:
Journal of virological methods
Volume:
187
Issue:
1
Pages:
138-143
Publication date:
2013-01-05
DOI:
EISSN:
1879-0984
ISSN:
0166-0934
URN:
uuid:aba285bf-7d87-4d7a-a7c9-3e891907f523
Source identifiers:
353947
Local pid:
pubs:353947

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