Journal article icon

Journal article

Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase.

Abstract:
We introduce a method for sequencing peptides by mass spectrometry using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When analyzed by electron transfer dissociation (ETD)-based mass spectrometric sequencing, Lys-N-digested peptides that contain a single lysine residue produce spectra dominated by c-type fragment ions, providing simple ladders for sequence determination. This method should be a valuable strategy for de novo sequencing and the analysis of post-translational modifications.
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1038/nmeth.1204

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
Journal:
Nature methods More from this journal
Volume:
5
Issue:
5
Pages:
405-407
Publication date:
2008-05-01
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Language:
English
Keywords:
Pubs id:
pubs:384605
UUID:
uuid:ab445972-f979-460d-a145-65737ba0407e
Local pid:
pubs:384605
Source identifiers:
384605
Deposit date:
2013-11-16

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP