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Effect of extracellular ph on matrix synthesis by chondrocytes in 3D agarose gel.

Abstract:

In cartilage tissue engineering, the determination of the most appropriate cell/tissue culture conditions to maximize extracellular matrix synthesis is of major importance. The extracellular pH plays an important role in affecting energy metabolism and matrix synthesis by chondrocytes. In this study, chondrocytes were isolated from bovine articular cartilage, embedded in agarose gel, and cultured at varied pH levels (7.3-6.6). Rate of lactate production, total glycosaminoglycan (GAG) and coll...

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Publication status:
Published

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Publisher copy:
10.1021/bp060024v

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Role:
Author
Journal:
Biotechnology progress
Volume:
23
Issue:
2
Pages:
430-434
Publication date:
2007-01-01
DOI:
EISSN:
1520-6033
ISSN:
8756-7938
URN:
uuid:aaf52dd0-6036-4f46-a84c-c2fb67628207
Source identifiers:
61771
Local pid:
pubs:61771

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