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Genome fingerprinting by pulsed-field gel electrophoresis of isolates of Burkholderia pseudomallei from patients with melioidosis in Thailand.

Abstract:

A total of 95 isolates of Burkholderia pseudomallei from 53 sporadic cases in Thailand were examined by pulsed-field gel electrophoresis. Digestion of genomic DNA of all isolates by NcoI generated a macrorestriction pattern similar to that of B. pseudomallei which cannot assimilate L-arabinose. Analysis using restriction enzymes SpeI and AvrII demonstrated greater sensitivity than NcoI digestion in the differentiation of B. pseudomallei and could be used for epidemiological groupings. Four cl...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Role:
Author
Host title:
Acta tropica
Volume:
74
Issue:
2-3
Pages:
187-191
Publication date:
2000-02-01
Event location:
NETHERLANDS
DOI:
EISSN:
1873-6254
ISSN:
0001-706X
Keywords:
Pubs id:
pubs:37245
UUID:
uuid:aabc0ad6-a962-4f13-8290-5cfa9d3087d2
Local pid:
pubs:37245
Source identifiers:
37245
Deposit date:
2012-12-19

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