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Characterization of a novel, stage-specific, invariant surface protein in Trypanosoma brucei containing an internal, serine-rich, repetitive motif.

Abstract:

A new surface membrane protein, invariant surface glycoprotein termed ISG100, was identified in Trypanosoma brucei, using catalyzed surface, radioiodination of intact cells. This integral membrane glycoprotein was purified by a combination of detergent extraction, lectin-affinity, and ion-exchange chromatography followed by preparative SDS-polyacrylamide gel electrophoresis. The protein was expressed only in bloodstream forms of the parasite, was heavily N-glycosylated, and was present in dif...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.272.46.29212

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Journal:
Journal of biological chemistry More from this journal
Volume:
272
Issue:
46
Pages:
29212-29221
Publication date:
1997-11-01
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
Language:
English
Keywords:
Pubs id:
pubs:102771
UUID:
uuid:aa7ffc90-d87e-4e7e-bbb1-999542a2aef2
Local pid:
pubs:102771
Source identifiers:
102771
Deposit date:
2012-12-19

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