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The accurate prediction of rare hemoglobin variants using a combination of high performance liquid chromatography, retention time and isoelectric focusing electrophoresis position.

Abstract:

OBJECTIVE: To investigate the predictive accuracy of using a combination of the high pressure liquid chromatography (HPLC) retention time and the relative isoelectric focusing (IEF) position to diagnose rare hemoglobin variants. METHODS: A selected group of 40 patients with a rare beta-chain variant were assigned a presumed diagnosis following HPLC and IEF screening and then the variant identified in each case by DNA analysis. The study was conducted at the National Hemoglobinopathy Referenc...

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Publication status:
Published

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Journal:
Saudi medical journal
Volume:
30
Issue:
9
Pages:
1158-1164
Publication date:
2009-09-05
EISSN:
1658-3175
ISSN:
0379-5284
URN:
uuid:aa442f0f-c8c6-4c1b-bba6-b0f0fd9473ed
Source identifiers:
164019
Local pid:
pubs:164019

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