Separation of monoclonal antibody alemtuzumab monomer and dimers using ultrafiltration.

Journal article

This article examines the feasibility of using ultrafiltration to separate the monomer of the monoclonal antibody alemtuzumab (Campath or Campath-1H) from a mixture of dimer and higher-order oligomers (collectively called "dimers" here). Using parameter scanning ultrafiltration, we initially assessed the suitability of the following membranes: 100 kDa and 300 kDa polyethersulfone (PES) membranes, and a 100 kDa polyvinylidene fluoride (PVDF) membrane. A detailed study was then carried out to e...

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Authors: Wan, Y; Vasan, S; Ghosh, R; Hale, G; Cui, Z

• Publication status: Published
• Journal: Biotechnology and bioengineering
• Volume: 90
• Issue: 4
• Pages: 422-432
• Publication date: 2005-05-01
• DOI: 10.1002/bit.20416
• EISSN: 1097-0290
• ISSN: 0006-3592
• Language: English
• Keywords: Antibodies, Monoclonal; Hydrogen-Ion Concentration; Antibodies, Monoclonal, Humanized; Salts; Antibodies, Neoplasm; Dimerization; Ultrafiltration