CD28 as a molecular amplifier extending TCR ligation and signaling capabilities.

Journal article

Evidence has gathered that CD28 costimulation facilitates T cell activation by potentiating TCR intrinsic-signaling. However, the underlying molecular mechanism is largely unknown. Here we show that, by enhancing T cell/APC close contacts, CD28 facilitates TCR signal transduction. Moreover, the signal supplied by CD28 does not lead to increased Zap-70 and Lat phosphorylation, but amplifies PLCgamma1 activation and Ca(2+) response. We provide evidence that the PTK Itk controls the latter funct...

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Authors: Michel, F; Attal-Bonnefoy, G; Mangino, G; Mise-Omata, S; Acuto, O

• Publication status: Published
• Journal: Immunity
• Volume: 15
• Issue: 6
• Pages: 935-945
• Publication date: 2001-12-01
• DOI: 10.1016/s1074-7613(01)00244-8
• EISSN: 1097-4180
• ISSN: 1074-7613
• Language: English
• Keywords: NFATC Transcription Factors; Carrier Proteins; Signal Transduction; CD4-Positive T-Lymphocytes; Humans; Membrane Proteins; Calcium Signaling; Transcription Factors; Transfection; Phosphorylation; Gene Expression Regulation; Type C Phospholipases; ZAP-70 Protein-Tyrosine Kinase; Protein-Tyrosine Kinases; Enzyme Activation; Phospholipase C gamma; Macromolecular Substances; DNA-Binding Proteins; Phosphoproteins; Antigens, CD80; Antigens, CD28; Lymphocyte Activation; Phosphatidylinositol 3-Kinases; Receptors, Antigen, T-Cell; Nuclear Proteins; Protein Processing, Post-Translational; Jurkat Cells; Sequence Deletion; Isoenzymes; Cell Line; Interleukin-2; Phosphotyrosine; Recombinant Fusion Proteins; Adaptor Proteins, Signal Transducing; Antigen-Presenting Cells