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Ribo-Pop: simple, cost-effective, and widely applicable ribosomal RNA depletion

Abstract:

The measurement of RNA abundance derived from massively parallel sequencing experiments is an essential technique. Methods that reduce ribosomal RNA levels are usually required prior to sequencing library construction because ribosomal RNA typically comprises the vast majority of a total RNA sample. For some experiments, ribosomal RNA depletion is favored over poly(A) selection because it offers a more inclusive representation of the transcriptome. However, methods to deplete ribosomal RNA ar...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1261/rna.076562.120

Authors


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Institution:
University of Oxford
Division:
MSD
Sub department:
Biochemistry
Role:
Author
ORCID:
0000-0002-4947-6048
More by this author
Role:
Author
ORCID:
0000-0003-1792-9206
More by this author
Institution:
University of Oxford
Division:
MSD
Sub department:
Biochemistry
Oxford college:
Jesus College
Role:
Author
ORCID:
0000-0002-5385-3053
Biotechnology and Biological Sciences Research Council More from this funder
Publisher:
Cold Spring Harbor Laboratory Publisher's website
Journal:
RNA Journal website
Volume:
26
Issue:
11
Pages:
1731-1742
Place of publication:
United States
Publication date:
2020-08-05
Acceptance date:
2020-07-31
DOI:
EISSN:
1469-9001
ISSN:
1355-8382
Pmid:
32759389
Language:
English
Keywords:
Pubs id:
1124784
Local pid:
pubs:1124784
Deposit date:
2020-12-08

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