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In vitro reconstitution of eukaryotic ion channels using droplet interface bilayers.

Abstract:

The ability to routinely study eukaryotic ion channels in a synthetic lipid environment would have a major impact on our understanding of how different lipids influence ion channel function. Here, we describe a straightforward, detergent-free method for the in vitro reconstitution of eukaryotic ion channels and ionotropic receptors into droplet interface bilayers and measure their electrical activity at both the macroscopic and single-channel level. We explore the general applicability of thi...

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Publication status:
Published

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Publisher copy:
10.1021/ja200128n

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Physics, Condensed Matter Physics
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Role:
Author
Journal:
Journal of the American Chemical Society
Volume:
133
Issue:
24
Pages:
9370-9375
Publication date:
2011-06-05
DOI:
EISSN:
1520-5126
ISSN:
0002-7863
URN:
uuid:a9af4552-2451-4c25-b485-1b6ba48ad5c4
Source identifiers:
140424
Local pid:
pubs:140424

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