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Characterization of the Na+, K(+)-ATPase in isolated bovine articular chondrocytes; molecular evidence for multiple alpha and beta isoforms.

Abstract:

We have used isoform-specific antibodies against the Na+, K(+)-ATPase alpha (alpha 1, alpha 2 and alpha 3) and beta (beta 1 and beta 2) subunit isoforms in order to establish their specific localization in isolated bovine articular chondrocytes. Immunoblotting confirmed the presence of the alpha 1 and alpha 3 isoforms, although alpha 1 expression was significantly greater than alpha 3 as assessed by immunofluorescence confocal laser scanning microscopy and PCR. A similar approach revealed the...

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Publication status:
Published

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Publisher copy:
10.1006/cbir.1997.0137

Authors


Mobasheri, A More by this author
Errington, RJ More by this author
Golding, S More by this author
Journal:
Cell biology international
Volume:
21
Issue:
4
Pages:
201-212
Publication date:
1997-04-05
DOI:
EISSN:
1095-8355
ISSN:
1065-6995
URN:
uuid:a99c1ec9-c70d-433b-a65f-fa860b7073d6
Source identifiers:
433365
Local pid:
pubs:433365

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