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A high-throughput alphavirus-based expression cloning system for mammalian cells.

Abstract:

We have developed a widely applicable functional genomics strategy based on alphavirus expression vectors. The technology allows for rapid identification of genes encoding a functional activity such as binding of a defined ligand. Complementary DNA (cDNA) libraries were expressed in mammalian cells following infection with recombinant Sindbis virus (SIN replicon particles), a member of the Alphavirus genus. Virus-infected cells that specifically bound a ligand of choice were isolated using fl...

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Publication status:
Published

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Publisher copy:
10.1038/nbt0901-851

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Journal:
Nature biotechnology More from this journal
Volume:
19
Issue:
9
Pages:
851-855
Publication date:
2001-09-01
DOI:
EISSN:
1546-1696
ISSN:
1087-0156

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