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Structure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts.

Abstract:
Structured noncoding RNAs underlie fundamental cellular processes, but determining their three-dimensional structures remains challenging. We demonstrate that integrating ¹H NMR chemical shift data with Rosetta de novo modeling can be used to consistently determine high-resolution RNA structures. On a benchmark set of 23 noncanonical RNA motifs, including 11 'blind' targets, chemical-shift Rosetta for RNA (CS-Rosetta-RNA) recovered experimental structures with high accuracy (0.6-2.0 Å all-heavy-atom r.m.s. deviation) in 18 cases.
Publication status:
Published

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Publisher copy:
10.1038/nmeth.2876

Authors


Sripakdeevong, P More by this author
Ziegeler, M More by this author
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Journal:
Nature methods
Volume:
11
Issue:
4
Pages:
413-416
Publication date:
2014-04-05
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:a95a6b31-65f9-4e0b-89b5-e7de3b8c349e
Source identifiers:
451046
Local pid:
pubs:451046

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