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Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection.

Abstract:

OBJECTIVE: To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca(2+) oscillations during activation. DESIGN: Test of a laboratory technique. SETTING: University medical school research laboratory. PATIENT(S): Donated unfertilized human oocytes from intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): Microinjection of oocytes with phospholipase C (PLC) zeta (ζ) cRNA and a Ca(2+)-sensitive fluorescent dye. MAIN OUTCOME ...

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Publication status:
Published

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Journal:
Fertility and sterility More from this journal
Volume:
97
Issue:
3
Pages:
742-747
Publication date:
2012-03-01
DOI:
EISSN:
1556-5653
ISSN:
0015-0282
Language:
English
Keywords:
Pubs id:
pubs:245919
UUID:
uuid:a943c88c-6c0f-4014-a870-78ebd48d2cac
Local pid:
pubs:245919
Source identifiers:
245919
Deposit date:
2013-11-16

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