Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection.

Journal article

OBJECTIVE: To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca(2+) oscillations during activation. DESIGN: Test of a laboratory technique. SETTING: University medical school research laboratory. PATIENT(S): Donated unfertilized human oocytes from intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): Microinjection of oocytes with phospholipase C (PLC) zeta (ζ) cRNA and a Ca(2+)-sensitive fluorescent dye. MAIN OUTCOME ...

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Authors: Swann, K; Windsor, S; Campbell, K; Elgmati, K; Nomikos, M

• Publication status: Published
• Journal: Fertility and sterility
• Volume: 97
• Issue: 3
• Pages: 742-747
• Publication date: 2012-03-01
• DOI: 10.1016/j.fertnstert.2011.12.013
• EISSN: 1556-5653
• ISSN: 0015-0282
• Language: English
• Keywords: Phosphoinositide Phospholipase C; Treatment Failure; Motion; Sperm Injections, Intracytoplasmic; Calcium Signaling; Oocytes; Cytoplasm; Microscopy, Fluorescence; Humans; Female; RNA, Complementary; Time Factors; Fluorescent Dyes; Male; Microinjections; Rheology