Role of the chaperone DnaK in protein solubility and conformational quality in inclusion body-forming Escherichia coli cells.
Misfolding-prone proteins produced in bacteria usually fail to adopt their native conformation and aggregate. In cells producing folding-reluctant protein species, folding modulators are supposed to be limiting, a fact that enhances protein deposition. Therefore, coproducing DnaK or other main chaperones along with the target protein has been a common approach to gain solubility, although with very inconsistent and often discouraging results. In an attempt to understand the reason for this in...Expand abstract
- Publisher copy:
- Copyright date: