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Role of the chaperone DnaK in protein solubility and conformational quality in inclusion body-forming Escherichia coli cells.

Abstract:

Misfolding-prone proteins produced in bacteria usually fail to adopt their native conformation and aggregate. In cells producing folding-reluctant protein species, folding modulators are supposed to be limiting, a fact that enhances protein deposition. Therefore, coproducing DnaK or other main chaperones along with the target protein has been a common approach to gain solubility, although with very inconsistent and often discouraging results. In an attempt to understand the reason for this in...

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Authors


Martínez-Alonso, M More by this author
Villaverde, A More by this author
Journal:
FEMS microbiology letters
Volume:
273
Issue:
2
Pages:
187-195
Publication date:
2007-08-05
DOI:
EISSN:
1574-6968
ISSN:
0378-1097
URN:
uuid:a8e8d02e-f2c7-4415-b137-498932b9963e
Source identifiers:
258388
Local pid:
pubs:258388

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