Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells

Journal article

The stochastic nature of generating eukaryotic transcripts challenges conventional methods for obtaining and analyzing single-cell gene expression data. In order to address the inherent noise, detailed methods are described on how to collect data on multiple genes in a large number of single cells using microfluidic arrays. As part of a study exploring the effect of genotype on Wnt pathway activation, data were collected for 96 qPCR assays on 1440 lymphoblastoid cells. The description of meth...

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Authors: Livak, K; Wills, Q; Tipping, A; Datta, K; Mittal, R

• Journal: Methods
• Volume: 59
• Issue: 1
• Pages: 71-79
• Publication date: 2013-01-01
• DOI: 10.1016/j.ymeth.2012.10.004
• EISSN: 1095-9130
• ISSN: 1046-2023
• Language: English
• Keywords: Single-cell gene expression profiling; Stochastic noise in gene expression; Real-time PCR; Microfluidic arrays; High throughput qPCR; Eukaryotic transcription