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Eukaryotic expression: developments for structural proteomics.

Abstract:

The production of sufficient quantities of protein is an essential prelude to a structure determination, but for many viral and human proteins this cannot be achieved using prokaryotic expression systems. Groups in the Structural Proteomics In Europe (SPINE) consortium have developed and implemented high-throughput (HTP) methodologies for cloning, expression screening and protein production in eukaryotic systems. Studies focused on three systems: yeast (Pichia pastoris and Saccharomyces cerev...

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Publication status:
Published

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Publisher copy:
10.1107/s0907444906029805

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
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Journal:
Acta crystallographica. Section D, Biological crystallography
Volume:
62
Issue:
Pt 10
Pages:
1114-1124
Publication date:
2006-10-01
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
Source identifiers:
19145
Language:
English
Keywords:
Pubs id:
pubs:19145
UUID:
uuid:a84ba634-898d-4bc6-8afd-f81dddc8b4ca
Local pid:
pubs:19145
Deposit date:
2012-12-19

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