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Electrochemical Recognition and Quantification of Cytochrome c Expression in Bacillus subtilis and aerobe/anaerobe Escherichia coli using N,N,N’,N’-tetramethyl-para-phenylene-diamine (TMPD)

Abstract:

The colorimetric identification of pathogenic and non-pathogenic bacteria in cell culture is commonly performed using the redox mediator N,N,N′,N′-tetramethyl-para-phenylene-diamine (TMPD) in the so-called oxidase test, which indicates the presence of bacterial cytochrome c oxidases. The presented study demonstrates the ability of electrochemistry to employ TMPD to detect bacteria and quantify the activity of bacterial cytochrome c oxidases. Cyclic voltammetry studies and chronoamperometry me...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1039/C7SC03498A

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Zoology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Physical & Theoretical Chem
Role:
Author
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chemical Science Journal website
Volume:
8
Issue:
11
Pages:
7682-7688
Publication date:
2017-09-20
Acceptance date:
2017-09-18
DOI:
EISSN:
2041-6539
ISSN:
2041-6520
Pubs id:
pubs:736353
UUID:
uuid:a83d7fb2-3273-4702-b7bd-65c0dbd94064
Local pid:
pubs:736353
Source identifiers:
736353
Deposit date:
2017-10-16

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