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THE EFFECT OF TEMPERATURE AND PROTEIN-CONTENT ON THE DISPERSIVE PROPERTIES OF BACTERIORHODOPSIN FROM H-HALOBIUM IN RECONSTITUTED DMPC COMPLEXES FREE OF ENDOGENOUS PURPLE MEMBRANE-LIPIDS - A FREEZE-FRACTURE ELECTRON-MICROSCOPY STUDY

Abstract:

Ten reconstituted bilayer complexes of bacteriorhodopsin from Halobacterium halobium and 1,2-dimyristoyl-sn-glycerol-3- phosphocholine with protein/lipid mole ratios of between 1:1440 and 1:67, have been entirely free (less than 0.02%) of endogenous purple membrane phospholipid as judged by 31P-NMR methods using Triton X-100 and cholate as detergents for solubilization of the protein and reconstitution by detergent dialysis. The nitroxide spin-label Tempo has been employed to determine the bi...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
Journal:
BIOCHIMICA ET BIOPHYSICA ACTA
Volume:
980
Issue:
1
Pages:
117-126
Publication date:
1989-03-27
DOI:
ISSN:
0006-3002
Source identifiers:
422184
Language:
English
Keywords:
Pubs id:
pubs:422184
UUID:
uuid:a818dd74-a3a0-43da-bf8f-53a3ece1f708
Local pid:
pubs:422184
Deposit date:
2013-11-17

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