Unique kinetics of nicotinic acid-adenine dinucleotide phosphate (NAADP) binding enhance the sensitivity of NAADP receptors for their ligand.

Journal article

Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a novel and potent Ca(2+)-mobilizing agent in sea urchin eggs and other cell types. Little is known, however, concerning the properties of the putative intracellular NAADP receptor. In the present study we have characterized NAADP binding sites in sea urchin egg homogenates. [(32)P]NAADP bound to a single class of high-affinity sites that were reversibly inhibited by NaCl but insensitive to pH and Ca(2+). Binding of [(32)P]NAADP was los...

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Authors: Patel, S; Churchill, G; Galione, A

• Publication status: Published
• Journal: Biochemical journal
• Volume: 352 Pt 3
• Issue: 3
• Pages: 725-729
• Publication date: 2000-12-01
• DOI: 10.1042/0264-6021:3520725
• EISSN: 1470-8728
• ISSN: 0264-6021
• Language: English
• Keywords: Ovum; NADP; Calcium Signaling; Cyclic ADP-Ribose; Hydrogen-Ion Concentration; Binding Sites; Animals; Kinetics; Ligands; Adenosine Diphosphate Ribose; Protein Binding; Calcium; Sodium Chloride; Sea Urchins; Inositol 1,4,5-Trisphosphate; Cell Extracts