Isolation of intact, functional mitochondria from the model plant Arabidopsis thaliana.

Journal article

The ability to isolate intact, functional mitochondria from plant tissues is a key technique in the study of the genome, proteome, and metabolic function of the plant mitochondrion. Traditionally, mitochondrial plant researchers have turned to specific plant systems and organs (such as potato tubers and pea shoots) from which mitochondria are readily isolated in large quantities. However, increasingly, research is focused on a small number of model species, and there is a need to adapt existi...

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Authors: Sweetlove, L; Taylor, N; Leaver, C

• Journal: Methods in molecular biology (Clifton, N.J.)
• Volume: 372
• Pages: 125-136
• Publication date: 2007-01-01
• DOI: 10.1007/978-1-59745-365-3_9
• EISSN: 1940-6029
• ISSN: 1064-3745
• Language: English
• Keywords: Hydroponics; Cell Fractionation; Mitochondria; Seedling; Cell Respiration; Arabidopsis; Models, Biological; Centrifugation