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Journal article

Auto-aligning stimulated emission depletion microscope using adaptive optics.

Abstract:
Stimulated emission depletion (STED) microscopy provides diffraction-unlimited resolution in fluorescence microscopy. Imaging at the nanoscale, however, requires precise alignment of the depletion and excitation laser foci of the STED microscope. We demonstrate here that adaptive optics can be implemented to automatically align STED and confocal images with a precision of 4.3 ± 2.3 nm.
Publication status:
Published

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Publisher copy:
10.1364/ol.38.001860

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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author
Journal:
Optics letters
Volume:
38
Issue:
11
Pages:
1860-1862
Publication date:
2013-06-01
DOI:
EISSN:
1539-4794
ISSN:
0146-9592
Source identifiers:
411140
Language:
English
Keywords:
Pubs id:
pubs:411140
UUID:
uuid:a7d89937-656b-4e57-abaf-60d809c36819
Local pid:
pubs:411140
Deposit date:
2013-11-17

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