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Dynamics of DNA replication: an ultrastructural study.

Abstract:

DNA replication in cells takes place in domains scattered throughout the nucleoplasm. We have characterized the dynamics of DNA synthesis in synchronized mid-S-phase HeLa cells. Saponin-permeabilized cells were allowed to elongate nascent DNA chains in presence of biotin-dUTP for 5, 15, and 30 min (a pulse experiment), or for 5 min followed by an incubation with unlabeled precursors for 10 or 25 min (a pulse-and-chase experiment). The replication foci were then identified in ultrathin section...

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Publication status:
Published

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Publisher copy:
10.1016/j.jsb.2004.08.001

Authors


Philimonenko, AA More by this author
Jackson, DA More by this author
Janácek, J More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
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Journal:
Journal of structural biology
Volume:
148
Issue:
3
Pages:
279-289
Publication date:
2004-12-05
DOI:
EISSN:
1095-8657
ISSN:
1047-8477
URN:
uuid:a7c1bf03-e809-433b-8c89-d902d4252ff4
Source identifiers:
8197
Local pid:
pubs:8197

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