- Abstract:
-
DNA replication in cells takes place in domains scattered throughout the nucleoplasm. We have characterized the dynamics of DNA synthesis in synchronized mid-S-phase HeLa cells. Saponin-permeabilized cells were allowed to elongate nascent DNA chains in presence of biotin-dUTP for 5, 15, and 30 min (a pulse experiment), or for 5 min followed by an incubation with unlabeled precursors for 10 or 25 min (a pulse-and-chase experiment). The replication foci were then identified in ultrathin section...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/j.jsb.2004.08.001
-
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- Journal:
- Journal of structural biology
- Volume:
- 148
- Issue:
- 3
- Pages:
- 279-289
- Publication date:
- 2004-12-05
- DOI:
- EISSN:
-
1095-8657
- ISSN:
-
1047-8477
- URN:
-
uuid:a7c1bf03-e809-433b-8c89-d902d4252ff4
- Source identifiers:
-
8197
- Local pid:
- pubs:8197
- Copyright date:
- 2004
Journal article
Dynamics of DNA replication: an ultrastructural study.
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