A novel method for measurement of submembrane ATP concentration.

Journal article

There has been considerable debate as to whether adenosine triphosphate (ATP) is compartmentalized within cells and, in particular, whether the ATP concentration directly beneath the plasma membrane, experienced by membrane proteins, is the same as that of the bulk cytoplasm. This issue has been difficult to address because there is no indicator of cytosolic ATP, such as those available for Ca(2+), capable of resolving the submembrane ATP concentration ([ATP](sm)) in real time within a single...

Expand abstract

Authors: Gribble, F; Loussouarn, G; Tucker, S; Zhao, C; Nichols, C

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 275
• Issue: 39
• Pages: 30046-30049
• Publication date: 2000-09-01
• DOI: 10.1074/jbc.m001010200
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Biosensing Techniques; Xenopus; Cell Membrane; Patch-Clamp Techniques; Oocytes; Mutation; Cytoplasm; Electric Conductivity; Potassium Channels, Inwardly Rectifying; Adenosine Triphosphate; Sequence Deletion; Potassium Channels; Animals; Cell Compartmentation