Journal article icon

Journal article

Robust CRISPR/Cas9 genome editing of the HUDEP-2 erythroid precursor line using plasmids and single stranded oligonucleotide donors

Abstract:

The study of cellular processes and gene regulation in terminal erythroid development has been greatly facilitated by the generation of an immortalised erythroid cell line derived from Human Umbilical Derived Erythroid Precursors, termed HUDEP-2 cells. The ability to efficiently genome edit HUDEP-2 cells and make clonal lines hugely expands their utility as the insertion of clinically relevant mutations allows study of potentially every genetic disease affecting red blood cell development. Ad...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

Actions


Access Document


Files:
Publisher copy:
10.3390/mps1030028

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; RDM Clinical Laboratory Sciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; RDM Clinical Laboratory Sciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Human Genetics Wt Centre
Role:
Author
Expand authors...
More from this funder
Funding agency for:
Cheong, PL
Expand funders...
Publisher:
MDPI Publisher's website
Journal:
Methods and Protocols Journal website
Volume:
1
Issue:
3
Pages:
Article: 28
Publication date:
2018-07-30
Acceptance date:
2018-07-23
DOI:
ISSN:
2409-9279
Pubs id:
pubs:904975
URN:
uri:a789be01-8a3e-4a98-b0e5-4fe4d027d2fa
UUID:
uuid:a789be01-8a3e-4a98-b0e5-4fe4d027d2fa
Local pid:
pubs:904975

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP