Secondary structure and assembly mechanism of an oligomeric channel protein.

Journal article

The alpha-toxin of Staphylococcus aureus is secreted as a water-soluble, monomeric polypeptide (Mr 33 182) that can assemble into an oligomeric membrane channel. By chemical cross-linking, we have confirmed that the major form of the channel is a hexamer. The circular dichroism spectrum of this hexamer in detergent revealed that it contains a high proportion of beta-sheet that we deduce must lie within the lipid bilayer when the protein is associated with membranes. The circular dichroism spe...

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Authors: Tobkes, N; Wallace, B; Bayley, H

• Publication status: Published
• Journal: Biochemistry
• Volume: 24
• Issue: 8
• Pages: 1915-1920
• Publication date: 1985-04-01
• DOI: 10.1021/bi00329a017
• EISSN: 1520-4995
• ISSN: 0006-2960
• Language: English
• Keywords: Lipid Bilayers; Protein Conformation; Circular Dichroism; Hemolysin Proteins; Molecular Weight; Peptide Fragments; Macromolecular Substances; Staphylococcus aureus; Electrophoresis, Polyacrylamide Gel; Bacterial Toxins